Author Topic: Microphotographs :D  (Read 1719 times)

  • Offline zpyder

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Microphotographs :D
on: March 07, 2010, 21:56:22 PM
Ive been a bit busy this weekend photographing tray 1 of 60 (:|) from the unis beetle collection.

On the plus side I think Im getting better at bespoke microphotography (or photomicrography if youre that way inclined)

A selection of the beetles Ive processed this weekend:

Carabus nitens


Carabus arvensis


Carabus hortensis


Cicindela campestris


Nebria salina


Carabus violaceous


Ive discovered what the mask tool is for in photoshop, so I can take multiple photos of different parts of a specimen at different focal depths, use the photomerge tool to align them, and then use masks to show only the in-focus parts :D

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  • Offline Clock'd 0Ne

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Re:Microphotographs :D
Reply #1 on: March 08, 2010, 01:31:03 AM
How are you getting them to stay still long enough? :lol:

  • Offline zpyder

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Re:Microphotographs :D
Reply #2 on: March 08, 2010, 07:37:02 AM
phnar phnar.

Glue and/or pins, posthumous...

  • Offline Serious

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Re:Microphotographs :D
Reply #3 on: March 08, 2010, 10:33:00 AM
You can also use layers to stick the photos on top of each other then rub through where its out of focus.

Also worth doing manual exposure if you can, set, it up for a brown beetle then try a black one. Might give better results. Or alter the exposure compensation a little.

If youre using an SLR to do this rather than your microscope then a ring flash is useful. Might be useful anyway. 7dayshop have an adequate one for about £100.

Microphotographs :D
Reply #4 on: March 14, 2010, 14:40:41 PM
sounds like it would be easier to just wind the aperture closed and use a longer exposure to get a lot more depth of field focusing the whole object...

  • Offline zpyder

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Re:Microphotographs :D
Reply #5 on: March 14, 2010, 17:39:29 PM
Quote from: Serious
You can also use layers to stick the photos on top of each other then rub through where its out of focus.


Already doing this...22 separate images I think this one was...


Exposure is also manual, I just played around a bit to find a decent ISO and shutter speed which doesnt get shake whilst using the available light. ISO 400 and a shutter speed of about 20 I think. Could go longer but would then need to fix the camera to the eyepiece and Ive yet to get the right t-adapter for it :D

Im not too keen on splashing out another load of money for a decent macro lens for the SLR, and technically I should be able to achieve a greater level of detail this way than I could with an expensive macro lens...


Re:Microphotographs :D
Reply #6 on: March 15, 2010, 18:04:53 PM
I think theres special software which can blend this kind of thing automatically for you (kind of like the panorama software you get, but for depth of field). Ive never tried it but might be worth a google.

Re:Microphotographs :D
Reply #7 on: March 15, 2010, 18:54:50 PM
ill see your beetles & raise you



no more beetles :D

  • Offline zpyder

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Re:Microphotographs :D
Reply #8 on: March 15, 2010, 19:52:27 PM
Quote from: Mongoose
I think theres special software which can blend this kind of thing automatically for you (kind of like the panorama software you get, but for depth of field). Ive never tried it but might be worth a google.


Aye, I think the useful ones though may be device specific? Certainly there are a couple of scopes at uni that have specific software for doing it. I think Hugin also can do it using "Enblend" or something. To be honest though I quite like the manualness of it, depending on the amount of detail I want it doesnt take too long at all to do. Certainly I think I probably spend less time doing them than some photographers would spend processing a normal photograph :D

Photoshop does a good job at merging the images, but it just gets confused over which ones to put on top. Once you have a method though its quite quick. Add the photos in the photomerge window, disable the blend option and merge them. This aligns them all. Add a mask to each layer and make all but the bottom one invisible. Now progressively make each layer above visible, in the process noting which bits are out of focus and drawing on the mask to remove them. Sounds like a lot of work but as the mask has a soft fade around the edge you can do a single layer in about 10-20 secs depending on detail...

  • Offline zpyder

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Re:Microphotographs :D
Reply #9 on: August 29, 2010, 23:18:27 PM
Well, time for a bit of an update.

Finally got a t-mount & adaptor for the microscope. To cut a long story short the original t-mount tube I got is just slightly the wrong size, but actually fits nicely on the 10x eyepiece on the trinoc. The one I got that I know WOULD fit, they sent the wrong one but actually is perfect for the trinoc without the eyepiece.

I also bit the bullet and paid up for HeliconFocus, which is one of those programs mongoose mentioned. It actually takes just as long for it to process as the manual PS way, the only difference being I can go away for 30 mins and come back and its done. The results seem to be alright too. Just dont like that its an annual licence. I might end up swapping to a different programme next year that is indefinite, unless I can get whoever employees me at the time to pay for it.

So:

~15mm long

Chlaenius nigricornis by Chris_Moody, on Flickr


~4-5mm long

Bembidion (Peryphus) femoratum by Chris_Moody, on Flickr


~5mm long

Porcellio scaber by Chris_Moody, on Flickr


Coastal woodlouse / isopod ~8mm long

Ligia oceanica by Chris_Moody, on Flickr

Re:Microphotographs :D
Reply #10 on: August 30, 2010, 00:21:52 AM
they scare me  :puke:

Microphotographs :D
Reply #11 on: August 30, 2010, 00:35:36 AM
Just checked one at original res on flickr, awesome magnification!

  • Offline zpyder

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Re:Microphotographs :D
Reply #12 on: August 30, 2010, 09:20:58 AM
Yeah, but they tend to get a bit soft at full res, or have CA, or something!

EDIT:
They have been shrunk down as well, they pretty much take up the whole of a photo, I dont know if that is 1:1 or whatever, those magnification scales confuse me.

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